5 Selective ribosome profiling

The selective ribosome profiling (SeRP) method is a new tool for studying the co-translational activity of maturation factors that provides proteome-wide information on a factor’s nascent interactome, the onset and duration of binding and the mechanisms controlling factor engagement. SeRP is based on the combination of two ribosome-profiling (RP) experiments, sequencing the ribosome-protected mRNA fragments from all ribosomes (total translatome) and the ribosome subpopulation engaged by the factor of interest (factor-bound translatome).

For more details see Selective ribosome profiling to study interactions of translating ribosomes in yeast.

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Schematic overview of SeRP:

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Analysis of eukaryotic selective RP data:

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